{"id":6487,"date":"2025-03-10T14:02:32","date_gmt":"2025-03-10T13:02:32","guid":{"rendered":"https:\/\/uni-freiburg.de\/frias\/?p=6487"},"modified":"2025-06-05T16:58:20","modified_gmt":"2025-06-05T14:58:20","slug":"prof-dr-harry-holthofer","status":"publish","type":"post","link":"https:\/\/uni-freiburg.de\/frias\/prof-dr-harry-holthofer\/","title":{"rendered":"Prof. Dr. Harry\u00a0Holth\u00f6fer"},"content":{"rendered":"\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns are-vertically-aligned-top is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-top is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<div class=\"wp-block-media-text is-stacked-on-mobile is-vertically-aligned-top\" style=\"grid-template-columns:40% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"200\" src=\"https:\/\/uni-freiburg.de\/frias\/wp-content\/uploads\/sites\/62\/Holthofer-Harry.png\" alt=\"Portrait of Harry Holth\u00f6fer\" class=\"wp-image-6488 size-full\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p>Dublin City University<br>Biomedicine<br><br>External Senior Fellow (Marie S. Curie FCFP)<br>January 2016 &#8211; March 2017<\/p>\n\n\n\n<div class=\"is-layout-flex wp-block-ufr-buttons-is-layout-flex\">\n\t\n\n<\/div>\n\n\n\n\n<p>E-Mail: <a href=\"mailto:harry.holthofer@helsinki.fi\">harry.holthofer@helsinki.fi<\/a><\/p>\n<\/div><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-top is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div class=\"[&amp;amp;&amp;gt;*]:bl-relative last:[&amp;amp;&amp;gt;*.bl-absolute]:bl-absolute\n\t\t\t[&amp;amp;&amp;gt;*]:bl-z-20 last:[&amp;amp;&amp;gt;*]:bl-z-10 bl-relative bl-overflow-x-clip\n\t\t\t[.wp-block-ufr-section_&amp;amp;]:bl-overflow-x-visible\n\t\t\tbl-bg-identity-lightblue bl-text-identity-black dark:bl-bg-identity-lightblue dark:bl-text-identity-black bl-py-[24px]  align wp-block-ufr-section has-global-padding is-layout-constrained wp-block-ufr-section-is-layout-constrained\">\n\n\t\n\n<p>Last Update: 31.08.2017<\/p>\n\n\t<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns are-vertically-aligned-center is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h2 class=\"wp-block-heading\">Curriculum Vitae<\/h2>\n\n\n\n<p><strong>Prof. Harry Holthofer&nbsp; MD, PhD<\/strong>&nbsp;graduated from the University of Helsinki,&nbsp; Finland and&nbsp; specialized in clinical microbiology\/immunology. His hospital-based positions have included those of internal medicine, pulmology, pediatrics and occupational health.<\/p>\n\n\n\n<p>The research of Prof Holthofer has focused on the biology of the glomerular filtration barrier, particularly in congenital nephrotic syndrome as well as kidney complications of&nbsp; diabetes. For that, various approaches ranging from molecular and cell biology, biochemistry combined with in vitro and in vivo analytical platforms have revealed new integral molecules constituting the functional kidney filtration barrier. The present main research line includes development of new methods for comprehensive understanding of urinary vesicles (including exosomes).<\/p>\n\n\n\n<p>He became Professor and Director of the Technomedicum Research Centre from 2001-2006 in Finland and subsequently moved to Ireland to take up the Professorship and Chair of Bioanalytical Sciences at Dublin City University and to become Director of the Center for Bioanalytical Sciences in September 2006.<\/p>\n\n\n\n<p>He has set up and directed several European Union (EU) funded programs focussing on diabetes, its biomarkers and new diagnostics and is presently setting up a wave of European collaborative grants to and from China.<\/p>\n\n\n\n<p>Prof Holthofer is a founding member of the International Committee to Standardise Proteomics Methods in Kidney Research and European Kidney Health Initiative and serves in the boards of several research organisations including the European ERA-EDTA and got several international prices and acknowledgements of his expertise and achievements.<\/p>\n\n\n\n<div style=\"height:32px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"bl-w-full wp-block-ufr-accordion\">\n\n\n<article class=\"ufr-accordion-container-wrapper\" data-accordion=\"animationDuration: 400; direction: vertical; collapsible: true; \">\n\t<div class=\"ufr-accordion-container-flex-wrapper \">\n\t\t\n\n<section\n\tid=\"Selected-Publications\"\n\tclass=\"ufr-accordion-item-section\n\tufr-accordion-item-section--vertical\">\n\t<div class=\"bl-group\/accordion-header ufr-accordion-item-header\n\t\t\t\"\n\trole=\"button\"\n\ttabindex=\"0\"\n\taria-label=\"Toggle accordion item: Selected Publications\"\n\taria-controls=\"Accordion Selected-Publications content\"\n\t>\n\t\t<div class=\"ufr-accordion-item-header-wrapper\n\t\t\t\t\t\">\n\t\t\t\t\t\t<h3 class=\"ufr-accordion-item-header-heading\n\t\t\t\tufr-accordion-item-header-heading--vertical\t\t\t\">\n\t\t\t\tSelected Publications\t\t\t<\/h3>\n\t\t<\/div>\n\t\t<span class=\"\n\t\t\tchevron-container\n\t\t\tufr-accordion-item-header-chevron-container\n\t\t\tufr-accordion-item-header-chevron-container--!numbered ufr-accordion-header-chevron-container--light-in-lightmode ufr-accordion-header-chevron-container--dark-in-darkmode \t\t\" aria-hidden=\"true\">\n\t\t\t<svg width=\"100%\" viewBox=\"0 0 35 50\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\n\taria-label=\"chevron-right\" class=\"bl-h-[40px] md:bl-h-[40px] xl:bl-h-[100px] bl-rotate-90\" role=\"img\">\n\t<title>chevron-right<\/title>\n\t<path d=\"M5.00012 5L25.6062 25.0002L5.00012 45\" stroke=\"currentColor\" stroke-width=\"12\" \/>\n<\/svg>\n\t\t<\/span>\n\t<\/div>\n\t<div\n\t\tid=\"Accordion Selected-Publications content\"\n\t\tclass=\"ufr-accordion-item-body\n\t\t\tufr-accordion-item-body--vertical\t\t\"\n\t\t\t>\n\t\t\t\t<div\n\t\tclass=\"ufr-accordion-item-body-outer-content-wrapper\n\t\t\t\t\t\"\n\t\taria-hidden=\"true\"\n\t\t>\n\t\t\t<div class=\"is-layout-flow\n\t\t\t\t\t\t\t\">\n\t\t\t\t\n\n<ul class=\"wp-block-list\">\n<li><strong>Heikkil\u00e4 E, Ristola M, Havana M,&nbsp;Holth\u00f6fer&nbsp;H<\/strong><strong>,&nbsp;<\/strong><strong>Lehtonen S:&nbsp;<\/strong><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21306299\" target=\"_blank\" rel=\"noreferrer noopener\">Trans-interaction of nephrin and Neph1\/Neph3 induces cell adhesion that associates with decreased tyrosine phosphorylation of nephrin.<\/a>&nbsp;Biochem J. 435:619-28, 2011<\/li>\n\n\n\n<li><strong>Li Y, Zhou C, Shao X, Liu X, Guo J, Zhang Y, Wang H, Wang X, Li B, Deng K, Liu Q,&nbsp;Holth\u00f6fer&nbsp;H, Zou H.<\/strong>&nbsp;<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24663403\" target=\"_blank\" rel=\"noreferrer noopener\">Hypertriglyceridemic waist phenotype and chronic kidney disease in a chinese population aged 40 years and older.<\/a>&nbsp;PLoS One. 2014, 9:e92322<\/li>\n\n\n\n<li><strong>Wasik AA, Koskelainen S, Hyv\u00f6nen ME, Musante L, Lehtonen E, Koskenniemi K, Tienari J, Vaheri A, Kerjaschki D, Szalay C, R\u00e9v\u00e9sz C, Varmanen P, Nyman TA, Hamar P,&nbsp;Holth\u00f6fer&nbsp;H, Lehtonen S.<\/strong>&nbsp;<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24726496\" target=\"_blank\" rel=\"noreferrer noopener\">Ezrin Is Down-Regulated in Diabetic Kidney Glomeruli and Regulates Actin Reorganization and Glucose Uptake via GLUT1 in Cultured Podocytes.<\/a>&nbsp;Am J Pathol. 2014,184:1727-39<\/li>\n\n\n\n<li><strong>Musante L, TataruchD, Gu D, Benito-Martin D, CalzaferriG, Aherne S, Holthofer H<\/strong>&nbsp;A Simplified Method to Recover Urinary Vesicles for Clinical Applications. Sci Rep. 2014 Dec 23;4:7532. doi: 10.1038\/srep07532<\/li>\n\n\n\n<li><strong>Ravid\u00e0 A, Kreivi M, Musante L, Miinalainen I,&nbsp; Byrne B, Saraswat M, Meleady P, ClynesM Holthofer H:<\/strong>Glycosylation Patterns in Rat Diabetic Nephropathy. Kidney Int. 2015 Jan 14. doi: 10.1038\/ki.2014.387<\/li>\n<\/ul>\n\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n<\/section>\n\n\n\n<section\n\tid=\"FRIAS-Project-2\"\n\tclass=\"ufr-accordion-item-section\n\tufr-accordion-item-section--vertical\">\n\t<div class=\"bl-group\/accordion-header ufr-accordion-item-header\n\t\t\t\"\n\trole=\"button\"\n\ttabindex=\"0\"\n\taria-label=\"Toggle accordion item: FRIAS Project\"\n\taria-controls=\"Accordion FRIAS-Project-2 content\"\n\t>\n\t\t<div class=\"ufr-accordion-item-header-wrapper\n\t\t\t\t\t\">\n\t\t\t\t\t\t<h3 class=\"ufr-accordion-item-header-heading\n\t\t\t\tufr-accordion-item-header-heading--vertical\t\t\t\">\n\t\t\t\tFRIAS Project\t\t\t<\/h3>\n\t\t<\/div>\n\t\t<span class=\"\n\t\t\tchevron-container\n\t\t\tufr-accordion-item-header-chevron-container\n\t\t\tufr-accordion-item-header-chevron-container--!numbered ufr-accordion-header-chevron-container--light-in-lightmode ufr-accordion-header-chevron-container--dark-in-darkmode \t\t\" aria-hidden=\"true\">\n\t\t\t<svg width=\"100%\" viewBox=\"0 0 35 50\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\n\taria-label=\"chevron-right\" class=\"bl-h-[40px] md:bl-h-[40px] xl:bl-h-[100px] bl-rotate-90\" role=\"img\">\n\t<title>chevron-right<\/title>\n\t<path d=\"M5.00012 5L25.6062 25.0002L5.00012 45\" stroke=\"currentColor\" stroke-width=\"12\" \/>\n<\/svg>\n\t\t<\/span>\n\t<\/div>\n\t<div\n\t\tid=\"Accordion FRIAS-Project-2 content\"\n\t\tclass=\"ufr-accordion-item-body\n\t\t\tufr-accordion-item-body--vertical\t\t\"\n\t\t\t>\n\t\t\t\t<div\n\t\tclass=\"ufr-accordion-item-body-outer-content-wrapper\n\t\t\t\t\t\"\n\t\taria-hidden=\"true\"\n\t\t>\n\t\t\t<div class=\"is-layout-flow\n\t\t\t\t\t\t\t\">\n\t\t\t\t\n\n<h3 class=\"wp-block-heading\"><strong>Urinary Exosomes for Novel Diabetes Diagnostics<\/strong><\/h3>\n\n\n\n<p>Proteinuria is a key manifestation of kidney diseases. It is a feared complication of diabetes and strongest predictor of cardiovascular emergencies. Glomerular epithelial cells,&nbsp;<strong><em>podocytes<\/em><\/strong>, are the main link between diabetes and proteinuria.&nbsp; These cells present with unique morphology, maintain body homeostasis and show huge vesicular transport activity.<\/p>\n\n\n\n<p><strong><em>Exosomes<\/em><\/strong>&nbsp;are recently found nano-microvesicles in many body fluids including urine, and contain a surprising variety of specific proteins, lipids and many distinct RNA species.<\/p>\n\n\n\n<p>Here we propose a multifaceted approach to reveal the urinary exosome (UE) signature in diabetes, with special emphasis on uncovering the fundamental mechanisms in the podocytes and whether their distinct protein and RNA&nbsp; signatures can be used&nbsp; as biomarkers.<\/p>\n\n\n\n<p>A variety of cell culture and existing experimental models will be compared in health vs. diabetic stress. Changes in UE transcriptomics,&nbsp; organellar proteomics and metabolomics will be collaboratively assayed with the equipment and top expertise readily available at the University of Freiburg. Advanced bioinformatics tools will be used to identify targets for potential monitoring and pathways involved in diabetes.<\/p>\n\n\n\n<p>Major breakthroughs in disease monitoring and diabetes management with significant practical consequences can be expected.<\/p>\n\n\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n<\/section>\n\n\t<\/div>\n<\/article>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<div class=\"[&amp;amp;&amp;gt;*]:bl-relative last:[&amp;amp;&amp;gt;*.bl-absolute]:bl-absolute\n\t\t\t[&amp;amp;&amp;gt;*]:bl-z-20 last:[&amp;amp;&amp;gt;*]:bl-z-10 bl-relative bl-overflow-x-clip\n\t\t\t[.wp-block-ufr-section_&amp;amp;]:bl-overflow-x-visible\n\t\t\tbl-bg-transparent bl-text-inherit dark:bl-bg-transparent dark:bl-text-inherit  alignfull wp-block-ufr-section has-global-padding is-layout-constrained wp-block-ufr-section-is-layout-constrained\">\n\n\t\t\t\t<div class=\"bl-absolute bl-inset-0 bl-w-full bl-h-full bl-pointer-events-none bl-mt-0\">\n\t\t\t<div\n\t\t\tclass=\"bl-absolute bl-origin-center\n\t\t\t\tbl-w-96\t\t\t\tbl-top-[50%] -bl-translate-y-[50%] bl-left-[50%] -bl-translate-x-[50%]\t\t\t\t\">\n\t\t\t\t<svg\n\twidth=\"100%\"\n\tviewBox=\"0 0 64.5 64.5\"\n\tfill=\"none\"\n\txmlns=\"http:\/\/www.w3.org\/2000\/svg\"\n\taria-label=\"\"\n\tclass=\"bl-relative bl-block dark:bl-hidden bl-w-full\"\n\trole=\"img\">\n\t<title><\/title>\n<linearGradient\n\tid=\"18LRkb6ctO\"\n\tx1=\"50%\" y1=\"100%\"\n\tx2=\"50%\" y2=\"0%\">\n\t<stop\n\t\toffset=\"0%\"\n\t\tstop-color=\"#f5c2ed\"\n\t\tstop-opacity=\"1\" \/>\n\t<stop\n\t\toffset=\"100%\"\n\t\tstop-color=\"#f5c2ed\"\n\t\tstop-opacity=\"0\" \/>\n<\/linearGradient>\n\t<path\n\t\td=\"m32.25,64c17.53,0,31.75-14.21,31.75-31.75S49.78.5,32.25.5.5,14.71.5,32.25s14.21,31.75,31.75,31.75\"\n\t\tstroke-width=\"2px\"\n\t\tvector-effect=\"non-scaling-stroke\"\n\t\tfill=\"url(#18LRkb6ctO)\" \/>\n<\/svg>\n<svg\n\twidth=\"100%\"\n\tviewBox=\"0 0 64.5 64.5\"\n\tfill=\"none\"\n\txmlns=\"http:\/\/www.w3.org\/2000\/svg\"\n\taria-label=\"\"\n\tclass=\"bl-relative bl-hidden dark:bl-block bl-w-full\"\n\trole=\"img\">\n\t<title><\/title>\n<linearGradient\n\tid=\"GcsLMAZSeX\"\n\tx1=\"50%\" y1=\"100%\"\n\tx2=\"50%\" y2=\"0%\">\n\t<stop\n\t\toffset=\"0%\"\n\t\tstop-color=\"#000149\"\n\t\tstop-opacity=\"1\" \/>\n\t<stop\n\t\toffset=\"100%\"\n\t\tstop-color=\"#000149\"\n\t\tstop-opacity=\"0\" \/>\n<\/linearGradient>\n\t<path\n\t\td=\"m32.25,64c17.53,0,31.75-14.21,31.75-31.75S49.78.5,32.25.5.5,14.71.5,32.25s14.21,31.75,31.75,31.75\"\n\t\tstroke-width=\"2px\"\n\t\tvector-effect=\"non-scaling-stroke\"\n\t\tfill=\"url(#GcsLMAZSeX)\" \/>\n<\/svg>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:128px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Dublin City UniversityBiomedicine External Senior Fellow (Marie S. Curie FCFP)January 2016 &#8211; March 2017 E-Mail: harry.holthofer@helsinki.fi Curriculum Vitae Prof. Harry Holthofer&nbsp; MD, PhD&nbsp;graduated from the University of Helsinki,&nbsp; Finland and&nbsp; specialized in clinical microbiology\/immunology. His hospital-based positions have included those of internal medicine, pulmology, pediatrics and occupational health. The research of Prof Holthofer has focused<\/p>\n","protected":false},"author":296,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_trash_the_other_posts":false,"editor_notices":[],"_show_in_pageentry_slider":true,"_pageentry_slider_title":"","footnotes":""},"categories":[53,351,26,350,89,132],"tags":[],"class_list":["post-6487","post","type-post","status-publish","format-standard","hentry","category-2016-17","category-dublin-city-university","category-fellow","category-ireland","category-marie-s-curie-frias-cofund-fellowship","category-medicine"],"featured_image_url":null,"featured_image_alt":"","_links":{"self":[{"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/posts\/6487","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/users\/296"}],"replies":[{"embeddable":true,"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/comments?post=6487"}],"version-history":[{"count":2,"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/posts\/6487\/revisions"}],"predecessor-version":[{"id":8396,"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/posts\/6487\/revisions\/8396"}],"wp:attachment":[{"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/media?parent=6487"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/categories?post=6487"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/uni-freiburg.de\/frias\/wp-json\/wp\/v2\/tags?post=6487"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}