Selected Publications
- Pirbadian, S., Barchinger, S. E., Leung, K. M., Byun, H. S., Jangir, Y., Bouhenni, R. A., Reed, S. B., Romine, M. F., Saffarini, D. A., Shi, L., Gorby, Y. A., Golbeck, J. H., and El-Naggar, M. Y. (2014) Shewanella oneidensis MR-1 nanowires are outer membrane and periplasmic extensions of the extracellular electron transport components, Proc Natl Acad Sci USA 111, 12883-12888.
- Saboe, P. O., Lubner, C. E., McCool, N. S., Vargas-Barbosa, N. M., Yan, H., Chan, S., Ferlez, B., Bazan, G. C., Golbeck, J. H., and Kumar, M. (2014) Two-Dimensional protein crystals for solar energy conversion, Adv Mater 26, 7064-7069.
- Gorka, M., Schartner, J., van der Est, A., Rogner, M., and Golbeck, J. H. (2014) Light-mediated hydrogen generation in Photosystem I: attachment of a naphthoquinone-molecular wire-Pt nanoparticle to the A1A and A1B sites, Biochemistry 53, 2295-2306.
- Srinivasan, N., Santabarbara, S, Rappaport, F., Carbonera, D., Redding, K., van der Est A. and Golbeck, J. (2011) “Alteration of the H-bond to the A1A phylloquinone in Photosystem I: influence on the kinetics and energetics of electron transfer”, J. Phys. Chem. B, 115, 1751-1759.
- Lubner, C. E., Applegate, A. M., Knörzer, P., Ganago, A., Bryant, D. A., Happe, T., and Golbeck, J. H. (2011) Solar hydrogen-producing bionanodevice outperforms natural photosynthesis, Proc. Natl. Acad. Sci. USA, 108, 20988-20991.
FRIAS Project
Single-Crystal ENDOR Studies of the Heliobacterial Reaction Center.
This project concerns a short-term (2 month) collaborative study between Prof. John Golbeck (Penn State University) and Prof. Stefan Weber (University of Freiburg). The first objective is the determination of the proton hyperfine tensors of P800+ by EPR/ENDOR spectroscopy on single crystals of the heliobacterial reaction center. This will be accomplished by studying the dependence of the ENDOR spectrum of the P800+ cation radical on the orientation of the crystal in the magnetic field. The proton hyperfine couplings from three of the methyl groups (positions 2, 7 and 12) attached to the BChl g ring are expected to be resolved, thereby allowing their principal values and corresponding axes orientations to be determined. A second objective is study of the conversion of BChl g to Chl a on the electronic properties of P800+. When the heliobacterial reaction center is exposed to light and oxygen, the 20 molecules of BChl g are spontaneously converted to Chl a. We will use use high-field EPR/ENDOR spectroscopy to decide whether the spin is localized on the BChl g molecule or on the Chl a molecule. From the orientation of the hyperfine tensors of the three methyl groups with respect to the crystallographic axis, the orientation whichever possesses the spin, the BChl g or Chl a molecule, can be determined. This project will provide the unprecedented opportunity to study the conversion of a homodimeric BChl g special pair to a heterodimeric BChl g/Chl a special pair all within the same protein framework.