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Equipment

Equipment and Instruments:

For detailed information on the equipment available at the Signalhaus and guidance on how to use them effectively, please explore the sections below and refer to our User Guidelines.

Measurements & Analysis

SpectraMax iD5 (Molecular Devices)

The SpectraMax iD5 Multi-Mode Microplate Reader comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization.

  • Fluorescence Filter: 360 nm, 400 nm, 425 nm, 485 nm
  • TRF (Em 350 nm; Ex 490 nm and 616 nm)
  • Fluorescence Polarization Filter: 465/35 nm

Synergy H4 Hybrid Microplate Reader (BioTek )

Multi-mode microplate reader the reads a 96-well microplate in 2 min.

  • Absorption: 96, 384, 1536 well plates
  • Fluorescence: 96 and 384 well plates
  • Luminescence: 96 and 384 well plates
  • FRET
  • TR-FRET
  • Fluorescence Polarization (Excitation: 360/40 nm, 485/20 nm, 540/25 nm and Emission: 460/40 nm, 528/40 nm, 620/40 nm)

BioTek EL406 Washer Dispenser

The combination of the BioTek EL406 Washer Dispenser, Thermo Scientific RapidSTAK plate stacker, and BioTek Synergy H4 microplate reader creates a powerful automated system for a wide range of liquid handling and analysis applications. This setup enables efficient, high-throughput processing from sample preparation to data acquisition.

  • Combination of 4 washing buffers and 3 dispenser reagents
  • Compatible with 96-, 384- and 1536-well plate
  • Automated for washing, dispensing and transport to plate reader
  • Plate stacker up to 30 microplates

Octet RED96 System (FortéBio)

The Octet RED96 is a label-free, real-time analysis instrument designed for characterization of protein-protein and protein-small molecule binding kinetics and for determining protein concentrations and titers.

  • Dip and Read™ label-free assay format
  • Uses disposable biosensors in an 8×12 array for simple, efficient assays
  • Measures the titer of 96 samples in just 30 minutes.
  • Kinetics: Association rate (ka), Dissociation rate (kd), Equilibrium constant (KD)

Zetasizer Nano S90 (Malvern)

The Zetasizer Nano S90 is a specialized instrument dedicated to the measurement of particle size and molecular weight. It utilizes Dynamic Light Scattering (DLS) technology with a fixed 90° detection angle.

  • Characterization of colloids and nanoparticles
  • Measures particles from 0.3 nm to 5 µm in diameter
  • Minimum Sample Volume: Requires only 20 μL of sample for measurements

Seahorse XFe96 Analyzer (Agilent)

The Seahorse XFe96 Analyzer is a powerful instrument for real-time metabolic analysis of live cells. It offers high-throughput capabilities and versatile applications in cellular metabolism research.

  • Measures both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in real-time.
  • Uses a 96-well plate format for efficient analysis of multiple samples.
  • Performs analysis without the need for fluorescent or radioactive labels.