Equipment

• ÄKTAexplorer
• ÄKTAxpress
• ÄKTAprime plus
• ÄKTA Avant 25

Our ÄKTA systems are equipped with a broad range of chromatographic columns and media, which cover all main types of chromatographic techniques: size exclusion, cation and anion exchange, affinity, hydrophobic interaction and immobilized-metal affinity (IMAC) chromatography.

The modules available in our laboratory include:

• Automated sampler
• UV/Vis detector
• Refractive index detector

• 4 lasers (488nm blue, 638nm red, 561nm yellow, 405 violet),
• 16 detection channels
• Flexible Sample Handling (standard FACS tubes, micro centrifuge tubes and 1.5 ml tubes)
• Attune NxT Autosampler compatible with 96-, 384-, and deep-well plates

• 3 excitation lines with independent, alignment-free focusing optics
• Simultaneous 9 color and 2 scatter parameters
• Analysis rates of 50,000 events per second
• Full 9 × 9 interlaser compensation matrix, and high sensitivity.

• Equipped with two lasers (488 nm, 561 nm) and four fluorescent detectors, plus forward and side scatters
• Complete internalized fluidics and temperature control system
• Two-way sorting (single, purity, enrich) with collection of up to 5 x 5 ml or 1.5 ml sample tube each direction

For detailed information about our service please refer to our Cell Sorting Service.

• Closed piezo-driven sorter system allows non-destructive cell sorting with high purity
• Sorting channels: GFP, PE, APC.

• Cooled CCD camera with 16-bit grayscale depth
• Luminescence measurements with very low background (<1%) in 20 min
• Max. Recording area 23×23 cm
• Lighting elements: UV table, UV incident light source, epi-LED incident light source in the visible range (blue, green, red)
• 580nm and 680 nm spectral filters for fluorescence measurements

• 700 nm and 800 nm fluorescence channels
• Multiplex fluorescence to detect two protein targets in each sample lane with great sensitivity
• Scan up to 9 mini-blots, 6 microplates, or 30 slides at the same time

• Our facility has two autoclavable benchtop cell culture bioreactors for culturing mammalian and insect cell lines.
• The CelliGen 310 (2.5 L and 14 L) can be operated in batch, fed-batch and perfusion mode independently of each other or connected in series.

• The 14 L serializable-in-place BioFlo 415 Fermentor is a robust production scale system, designed to meet any research and production needs.
• Up to 32 parameters can be controlled by the BioCommand software, which allows very high cell density growth.

Nucleofector® II device is a tool for highly efficient non-viral transfection of primary cells and difficult-to-transfect cell lines

Gene Pulser MXcell systems can be used to electroporate as few as 10⁵ cells by using a 96-well plate or as many as 10⁶ to 10_⁷ cells in a 12-well plate or in a cuvette.

The Multiporator can be used for electroporation of animal and human cells, primary cells, plant cells and embryonic stem cells. The system provides multiple pulsing from 1 to 99 pulses with a one-minute interval.

For transformation of bacteria, yeast, and other microorganism. Manual programming allows voltage to be selected in a 200–3,000 V range with 10 V precision and allows pulse width to be selected in a 1.0–4.0 ms range with 0.1 ms precision

• High-performance real-time PCR instrument high-throughput sample processing in a 384-well format
• Sensitive 4-Color detection optics allows for multiplex reactions with up to 4 targets

• The freeze dryer ALPHA 1-4 LSC is a high performance universal laboratory unit for freeze drying of solid or liquid products in ampoules, vials, glass, flasks, plasma bottles or dishes.
• The LSCplus controller supports manual and automatic process control for reproducible results.

• The TS 1.1 KW homogenizer is a bench-top model that processed all kind of cell suspensions.
• Efficiently disrupts: bacteria, yeast, algae, mammalian and insect cells, fungus etc.

• SONOPULS ultrasonic homogenizers generate high-performance ultrasound with high intensities and ultrasonic amplitudes
• For disruption of cell, bacteria or tissue, production of emulsions or homogenization of substances
• HD2070 located at room temperature
• HD3100 is permanently located in the 4°C cold room, ideal for sensitive sample preparation
• Volumes between 1 ml and 500 ml

• The Zepto plasma system can be used for process development, cleaning, activation and etching in small batches of different materials, such as glass, metal, plastics, ceramic.

Robotic setup:

• 8-channel disposable tips, pipetting: 1-1000 µl
• Heated incubator up to 37°C without CO₂
• Carriers can be adjusted for individual temperature (FL601 Recirculating Cooler: -20°C to +40°C)
• Carousel with stacking system for 110 plates, 21 racks of 10-200 µl and 12 racks of 1000 µl disposable tips
• BioTek 405^TM TSUVS plate washer for 96- and 384-well microplates

BioTek Multiflo Microplate Dispenser:

• Automated multimode reagent dispenser for 96-, 384- and 1536-well microplates
• Cell-friendly angled dispense tips
• Dispense a 384-well microplate in 6 seconds with a minimum volume of 1 µl

Robotic setup:

• 8-channel fixed tips
• CO₂ incubator with carousel for 15 independent LED light boxes at 490, 660, 740 nm wavelength
• Carrier adjustable to 2 different temperatures (e.g. +4 and +37 °C)
• Centrifuge for micoplates (Hettich ROTANTA 460 Robotic)
• Flow cytometer for 96-well microplates

Liconic Worktable Incubator:

• 2 slots can run in parallel
• Temperature: RT or 37 °C
• LED light: 490, 660, 740 nm
• No connection for CO₂

Liconic CO2 incubator with LED:

• 15 slots can run in parallel with different light programmes
• Temperature: 37 °C
• LED light: 490, 660, 740 nm
• Connection to CO₂

MACSQuant^® Analyzer 10 Flow Cytometer:

• Lasers: 405, 488, 638 nm
• 10 optical channels (FSC, SSC and eight fluorescence channels) for multi-parameter flow cytometry
• Multi-sample analysis of 96-wells microplates
• Integrated auto-labelling and MACSQuant column for magnetic rare cell pre-enrichment