Seal element of the university of freiburg in the shape of a clover

Equipment

For detailed information on the equipment available at the Signalhaus and guidance on how to use them effectively, please explore the sections below and refer to our User Guidelines.

Measurements & Analysis

SpectraMax iD5 (Molecular Devices)

The SpectraMax iD5 Multi-Mode Microplate Reader comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization.

  • Fluorescence Filter: 360 nm, 400 nm, 425 nm, 485 nm
  • TRF (Em 350 nm; Ex 490 nm and 616 nm)
  • Fluorescence Polarization Filter: 465/35 nm

Synergy H4 Hybrid Microplate Reader (BioTek )

Multi-mode microplate reader the reads a 96-well microplate in 2 min.

  • Absorption: 96, 384, 1536 well plates
  • Fluorescence: 96 and 384 well plates
  • Luminescence: 96 and 384 well plates
  • FRET
  • TR-FRET
  • Fluorescence Polarization (Excitation: 360/40 nm, 485/20 nm, 540/25 nm and Emission: 460/40 nm, 528/40 nm, 620/40 nm)

BioTek EL406 Washer Dispenser

The combination of the BioTek EL406 Washer Dispenser, Thermo Scientific RapidSTAK plate stacker, and BioTek Synergy H4 microplate reader creates a powerful automated system for a wide range of liquid handling and analysis applications. This setup enables efficient, high-throughput processing from sample preparation to data acquisition.

  • Combination of 4 washing buffers and 3 dispenser reagents
  • Compatible with 96-, 384- and 1536-well plate
  • Automated for washing, dispensing and transport to plate reader
  • Plate stacker up to 30 microplates

Octet RED96 System (FortéBio)

The Octet RED96 is a label-free, real-time analysis instrument designed for characterization of protein-protein and protein-small molecule binding kinetics and for determining protein concentrations and titers.

  • Dip and Read™ label-free assay format
  • Uses disposable biosensors in an 8×12 array for simple, efficient assays
  • Measures the titer of 96 samples in just 30 minutes.
  • Kinetics: Association rate (ka), Dissociation rate (kd), Equilibrium constant (KD)

Zetasizer Nano S90 (Malvern)

The Zetasizer Nano S90 is a specialized instrument dedicated to the measurement of particle size and molecular weight. It utilizes Dynamic Light Scattering (DLS) technology with a fixed 90° detection angle.

  • Characterization of colloids and nanoparticles
  • Measures particles from 0.3 nm to 5 µm in diameter
  • Minimum Sample Volume: Requires only 20 μL of sample for measurements

Seahorse XFe96 Analyzer (Agilent)

The Seahorse XFe96 Analyzer is a powerful instrument for real-time metabolic analysis of live cells. It offers high-throughput capabilities and versatile applications in cellular metabolism research.

  • Measures both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in real-time.
  • Uses a 96-well plate format for efficient analysis of multiple samples.
  • Performs analysis without the need for fluorescent or radioactive labels.